Aerial view of campus with Williamsport, the Susquehanna River and Bald Eagle Mountain as a backdrop

High Pressure Liquid Chromatography (HPLC)

hplc

Aaron McGaw eagerly anticipates his HPLC results.

High Pressure Liquid Chromatography (HPLC) is an important separation technique for compounds of low or no volatility thus it makes a nice complimentary procedure to GC. Like GC and all other chromatographic techniques, in HPLC the separation is based on a partitioning of the sample components on a stationary phase and a mobile phase. The liquid mobile phase is the variable. Both the polarity and pH of the mobile phase can be varied. We possess a Waters 510 HPLC equipped with a Waters 991 UV-VIS photodiode array detector. This equipment was obtained with the aid of a 1995 National Science Foundation Grant written by Dr. Franz.